高效液相色谱-荧光检测法测定尿液和血清中的苯丙酮酸。

Determination of phenylpyruvic acid in urine and serum by high-performance liquid chromatography with fluorescence detection.

作者信息

Hirata T, Kai M, Kohashi K, Ohkura Y

出版信息

J Chromatogr. 1981 Nov 13;226(1):25-31. doi: 10.1016/s0378-4347(00)84202-4.

PMID:7320149

Abstract

A highly sensitive and simple method for the determination of phenylpyruvic acid in urine and serum is described which employs high-performance liquid chromatography with fluorescence detection. Phenylpyruvic acid, after extraction with ethyl acetate, is reacted with 4'-hydrazino-2-stilbazole in aqueous methanol to give the corresponding fluorescent hydrazone which is separated by reversed-phase chromatography on muBondapak Phenyl. The lower limits of detection are 25 and 32 pmol for phenylpyruvic acid in 0.2 ml of urine and serum, respectively. This sensitivity permits the determination of the acid in urine or normal adults and newborn infants.

摘要

本文描述了一种用于测定尿液和血清中苯丙酮酸的高灵敏度且简便的方法，该方法采用带荧光检测的高效液相色谱法。苯丙酮酸经乙酸乙酯萃取后，在甲醇水溶液中与4'-肼基-2-芪唑反应，生成相应的荧光腙，然后在μBondapak苯基反相色谱柱上进行分离。对于0.2 ml尿液和血清中的苯丙酮酸，检测下限分别为25和32 pmol。这种灵敏度使得能够测定正常成年人和新生儿尿液中的该酸。