聚乙二醇用于非沉淀抗体定量测定的适用性评估。
Evaluation of polyethylene glycol's suitability for quantitative determination of non-precipitating antibody.
作者信息
Habeeb A F
出版信息
J Immunol Methods. 1981;47(2):209-17. doi: 10.1016/0022-1759(81)90121-6.
Polyethylene glycol (PEG) has been used extensively in the quantitative precipitin reaction and for the determination of soluble immune complexes. It has now been found that the optimum concentration of PEG for precipitation of non-precipitating antibodies in the quantitative precipitin reaction varies with the dilution of antisera. Immune precipitates obtained with PEG were examined for their protein content by polyacrylamide gel electrophoresis to detect non-specific precipitation of serum proteins. Determination of non-precipitating antibodies in sera of goats and pigs immunized with chicken egg-white lysozyme by the quantitative precipitin reaction in the presence of PEG, yielded lower values than obtained by immunoabsorbents. Care should be exercised in interpreting results when PEG is used to enhance precipitation.
聚乙二醇（PEG）已广泛应用于定量沉淀反应及可溶性免疫复合物的测定。现已发现，在定量沉淀反应中用于沉淀非沉淀性抗体的PEG最佳浓度会因抗血清的稀释度而异。通过聚丙烯酰胺凝胶电泳检测用PEG获得的免疫沉淀物的蛋白质含量，以检测血清蛋白的非特异性沉淀。在用PEG存在下通过定量沉淀反应测定用鸡蛋白溶菌酶免疫的山羊和猪血清中的非沉淀性抗体时，得到的值低于用免疫吸附剂获得的值。当使用PEG增强沉淀时，在解释结果时应谨慎。
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