一种用于生产低交叉反应性抗胆囊收缩素特异性抗血清的新型免疫程序。
A novel immunization procedure for production of anti-cholecystokinin-specific antiserum of low cross-reactivity.
作者信息
Tateishi K, Hamaoka T, Sugiura N, Yanaihara C, Yanaihara N
出版信息
J Immunol Methods. 1981;47(2):249-58. doi: 10.1016/0022-1759(81)90125-3.
PMID:7320512
Abstract
An anti-cholecystokinin octapeptide (CCK-8) antibody without cross-reactivity with pentagastrin, whose amino acid sequence is identical with the carboxy-terminal portion of CCK-8, was produced by immunization with CCK-8-keyhole limpet hemocyanin and injection of a conjugate of pentagastrin and a copolymer of D-glutamic acid and D-lysine to inhibit production of antibody cross-reacting with pentagastrin.
摘要
一种与五肽胃泌素无交叉反应的抗胆囊收缩素八肽（CCK-8）抗体被制备出来，其氨基酸序列与CCK-8的羧基末端部分相同。该抗体是通过用CCK-8-钥孔戚血蓝蛋白免疫，并注射五肽胃泌素与D-谷氨酸和D-赖氨酸共聚物的缀合物来抑制与五肽胃泌素发生交叉反应的抗体产生而制备的。
Zotero 插件