West D P, Fillenz M
J Neurochem. 1981 Oct;37(4):1052-3. doi: 10.1111/j.1471-4159.1981.tb04496.x.
Potassium-evoked tritiated noradrenaline (NA) release from hippocampal synaptosomes was measured with a superfusion method. A single 2-min high-K+ pulse released 39% of the vesicular NA by a Ca2+-dependent mechanism: the Ca2+-independent release was negligible. After changing the vesicular NA store size by pretreating rats with either alpha-methyl-para-tyrosine, 500 mg/kg, or tranylcypromine, 10 mg/kg, a single K+ pulse released a constant percentage of the vesicular NA. With two K+ pulses, however, there was a reduction in the percentage of vesicular NA released in response to the second pulse.
采用灌流法测定钾离子诱发的海马突触体中氚标记去甲肾上腺素（NA）的释放。单次2分钟的高钾脉冲通过钙离子依赖机制释放了39%的囊泡内NA：非钙离子依赖的释放可忽略不计。在用500mg/kg的α-甲基对酪氨酸或10mg/kg的反苯环丙胺预处理大鼠以改变囊泡内NA储存量后，单次钾离子脉冲释放出囊泡内NA的恒定百分比。然而，给予两个钾离子脉冲时，对第二个脉冲反应释放的囊泡内NA百分比有所降低。
