Hart D T, Vickerman K, Coombs G H
Mol Biochem Parasitol. 1981 Nov;4(1-2):39-51. doi: 10.1016/0166-6851(81)90027-x.
Promastigotes of Leishmania mexicana mexicana recently derived from amastigotes by transformation in vitro respired at a rate (17 nmol O2/min per 10(8) parasites) 4-5 times higher than that of amastigotes, but when the difference in cell protein content between the two preparations was taken into account the rates were not significantly different (32 nmol O2/min per mg protein). The respiration of both amastigotes and promastigotes was sensitive to cyanide, azide, antimycin A, 2-n-heptyl-4-hydroxyquinoline-N-oxide and high concentrations of amytal, but insensitive to rotenone and salicyl-hydroxamic acid, indicating that the two developmental forms possess a similar cytochrome-containing respiratory chain. D-Glucose and non-esterified fatty acids stimulated promastigote respiration and amastigote transformation to promastigotes in vitro; possibly these substances are important exogenous energy substrates for both forms of the parasites. Amino acids (incuding L-proline) and proteins did not appear to be used as energy substrates. The respiration rate of promastigotes was found to rise significantly upon continued sub-culture in vitro; at the same time cell size and protein content increased.
最近通过体外转化从无鞭毛体获得的墨西哥利什曼原虫前鞭毛体的呼吸速率（每10⁸个寄生虫17 nmol O₂/分钟）比无鞭毛体高4至5倍，但考虑到两种制剂之间细胞蛋白质含量的差异，呼吸速率并无显著差异（每毫克蛋白质32 nmol O₂/分钟）。无鞭毛体和前鞭毛体的呼吸均对氰化物、叠氮化物、抗霉素A、2-正庚基-4-羟基喹啉-N-氧化物和高浓度的戊巴比妥敏感，但对鱼藤酮和水杨基羟肟酸不敏感，这表明这两种发育形式具有相似的含细胞色素呼吸链。D-葡萄糖和非酯化脂肪酸在体外刺激前鞭毛体呼吸和无鞭毛体向前鞭毛体的转化；这些物质可能是两种形式寄生虫重要的外源性能量底物。氨基酸（包括L-脯氨酸）和蛋白质似乎未被用作能量底物。发现前鞭毛体在体外持续传代培养时呼吸速率显著上升；同时细胞大小和蛋白质含量增加。
