Fukuda J, Yamaguchi K
Neurosci Lett. 1981 Nov 4;26(3):263-8. doi: 10.1016/0304-3940(81)90143-9.
Nerve cells were isolated from dorsal root ganglia of senescent mouse (C57 black, male) by collagenase and were grown on collagen-coated plastic dishes. The nerve cells extended their neurites and survived for more than 3 weeks in vitro. Intracellular recording with a glass microelectrode revealed that, although their resting membrane potential was small, the tissue-cultured nerve cells recovered membrane excitability after continuous hyperpolarization by DC current passage. Both Na and Ca spikes were elicited in thus hyperpolarized nerve cells. Some nerve cells generated tetrodotoxin-resistant Na spikes.
通过胶原酶从衰老小鼠（C57 黑，雄性）的背根神经节中分离出神经细胞，并将其培养在涂有胶原蛋白的塑料培养皿上。神经细胞伸出神经突并在体外存活超过 3 周。用玻璃微电极进行细胞内记录显示，尽管其静息膜电位较小，但经直流电流持续超极化后，组织培养的神经细胞恢复了膜兴奋性。在如此超极化的神经细胞中引发了 Na 和 Ca 峰电位。一些神经细胞产生了对河豚毒素耐受的 Na 峰电位。
