胱氨酸病成纤维细胞在酸性pH介质中游离胱氨酸会减少。

Cystinotic fibroblasts are depleted of free-cystine by acid pH medium.

作者信息

Ritchie D G, Jonas A J, Oshima R G, Neal P, Schneider J A

出版信息

Pediatr Res. 1981 Dec;15(12):1492-4. doi: 10.1203/00006450-198112000-00007.

DOI:10.1203/00006450-198112000-00007

Abstract

The free (nonprotein) cystine content of human cystinotic fibroblasts was found to vary with the pH of their culture medium. Intracellular cystine content was highest at alkaline medium pH's and lowest at acidic medium pH's. When maintained in medium of pH 6.3, cystinotic fibroblasts lost 50% of their free-cystine content in 6 hr and over 95% in 30 hr. Cystine was the only amino acid to show significant variation with changes in the pH of the culture medium.

摘要

研究发现，胱氨酸病患者的成纤维细胞中游离（非蛋白质）胱氨酸含量会随培养基的pH值变化而改变。细胞内胱氨酸含量在碱性培养基pH值条件下最高，在酸性培养基pH值条件下最低。当培养于pH值为6.3的培养基中时，胱氨酸病患者的成纤维细胞在6小时内失去了50%的游离胱氨酸含量，在30小时内失去了超过95%。胱氨酸是唯一一种随培养基pH值变化而呈现显著变化的氨基酸。