Navarrete M H, Sanz-Zamarro M L
Rev Esp Fisiol. 1981 Sep;37(3):331-4.
Soluble polypeptides and their biosynthesis rate have been studied in root meristems of Allium cepa L. bulbs by electrophoresis in SDS polyacrylamide gels. The second mm of the root was chosen in all cases. The polypeptide composition of root meristems growing with a steady state kinetics at 15 degrees C and 25 degrees C appears to be roughly constant. The quantitative differences observed may be due to the different proportion of proliferative cells in the root segments studied, being higher at 15 degrees C than at 25 degrees C. One important fact is the existence of a polypeptide, approximately 80,000 daltons in molecular weight, which appears as an indicator of active proliferation in onion root cells.
通过SDS聚丙烯酰胺凝胶电泳,对洋葱鳞茎根分生组织中的可溶性多肽及其生物合成速率进行了研究。在所有情况下均选取根的第二个毫米段。在15摄氏度和25摄氏度下以稳态动力学生长的根分生组织的多肽组成似乎大致恒定。观察到的定量差异可能是由于所研究根段中增殖细胞的比例不同,15摄氏度时的比例高于25摄氏度时。一个重要的事实是存在一种分子量约为80,000道尔顿的多肽,它似乎是洋葱根细胞中活跃增殖的指标。