膜透析和过滤除菌对促红细胞生成素活性的影响。
Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
作者信息
Gallicchio V S, Murphy M J
出版信息
Yale J Biol Med. 1981 Jul-Aug;54(4):249-54.
Most erythropoietin (Ep) preparations contain non-erythropoietin contaminants. The use of such hormone concentrates raises important questions regarding interpretations of results derived from in vivo and especially from in vitro studies. By sterilizing various Ep preparations with Nalgene, Millipore, or Selas silver filtration, or even after conventional membrane dialysis, variable responses were noted when the Ep was assayed with mouse bone marrow cells in vitro (i.e. by stimulating the production of erythroid colonies from CFU-e and BFU-e) and in vivo (i.e., by using the exhypoxic, polycythemic mouse bioassay for Ep). The utility and limitations of such preparative procedures are discussed.
大多数促红细胞生成素（Ep）制剂都含有非促红细胞生成素污染物。使用这类激素浓缩物引发了一些关于体内研究尤其是体外研究结果解读的重要问题。通过用Nalgene、Millipore或Selas银滤器对各种Ep制剂进行灭菌，甚至在常规膜透析后，当在体外（即通过刺激CFU-e和BFU-e产生红系集落）和体内（即通过使用低氧、红细胞增多小鼠生物测定法检测Ep）用小鼠骨髓细胞检测Ep时，会观察到不同的反应。本文讨论了这些制备程序的实用性和局限性。
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