“欧洲冷杉”凋落物微生物（细菌和酵母菌）几丁质酶活性的测定（作者译）

[Determination of the chitinolytic activity of "Abies alba" Mill. litter microorganisms: bacteria and yeasts (author's transl)].

作者信息

Faure-Raynaud M

出版信息

Ann Microbiol (Paris). 1981 Sep-Oct;132B(2):267-79.

Abstract

Chitin decomposition by some microorganisms isolated from Abies alba Mill. litter has been observed. The active strains were representatives of the genera Bacillus, Pseudomonas and Streptomyces. The isolated yeasts were unable to decompose chitin. The chitinolytic microorganisms secreted in medium an extracellular chitinase system which determined chitin hydrolysis. Cell-free culture filtrate of the microorganisms produced colloidal chitin hydrolysis. An estimation method of chitin hydrolysis was determined measuring chitin loss in standard conditions. Experimental results indicate that Pseudomonas isolate is the most active. The active strains represent about 15% of the microorganisms, bacteria and yeasts, isolated from this litter.

摘要

已观察到从欧洲冷杉凋落物中分离出的一些微生物对几丁质的分解作用。活性菌株为芽孢杆菌属、假单胞菌属和链霉菌属的代表。分离出的酵母无法分解几丁质。几丁质分解微生物在培养基中分泌一种细胞外几丁质酶系统，该系统决定了几丁质的水解。微生物的无细胞培养滤液产生了胶体几丁质水解。通过测量标准条件下几丁质的损失确定了一种几丁质水解的估算方法。实验结果表明，分离出的假单胞菌活性最高。活性菌株约占从该凋落物中分离出的微生物（细菌和酵母）的15%。