Carrillo L, Gómez Molina S E
Facultad de Ciencias Agrarias, Universidad Nacional de Jujuy, Argentina.
Rev Argent Microbiol. 1998 Apr-Jun;30(2):73-8.
Twenty nine mesophilic and 19 thermophilic actinomycetes from sugar cane phyllosphere, rhizosphere and root-free soil were isolated. Twelve mesophilic and 7 thermophilic strains produced clear zones of hydrolyses on colloidal chitin agar and hydrolysed a chitin colloidal suspension. The rhizosphere strains were significantly more chitinolytic (p < 0.05) than the root-free soil strains. Streptomyces griseoruber 202 produced chitinase in colloidal chitin liquid medium with an activity of 108 micrograms of colloidal chitin hydrolysed/mg protein/hour, at pH 4.6 and 40 degrees C. This strain also produced chitinase in a medium with insect exoskeleton powder.
从甘蔗叶际、根际和无根土壤中分离出29株嗜温放线菌和19株嗜热放线菌。12株嗜温菌株和7株嗜热菌株在胶体几丁质琼脂上产生了水解透明圈，并能水解几丁质胶体悬浮液。根际菌株的几丁质分解能力显著高于无根土壤菌株（p < 0.05）。灰色链霉菌202在胶体几丁质液体培养基中产生几丁质酶，在pH 4.6和40℃条件下，其活性为每毫克蛋白质每小时水解108微克胶体几丁质。该菌株在含有昆虫外骨骼粉末的培养基中也能产生几丁质酶。
