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[用于评估改良蛋白质的猪的营养与生理研究。2. 采用¹⁵N示踪法测定小肠内容物中的内源氮]

[Nutritional and physiological studies of swine for the evaluation of modified proteins. 2. Determination of endogenous N in the contents of the small intestines using the 15N-tracer method].

作者信息

Souffrant W B, Köhler R, Matkowitz R, Gebhardt G, Schmandke H

出版信息

Arch Tierernahr. 1981 Oct;31(10):675-83. doi: 10.1080/17450398109426876.

DOI:10.1080/17450398109426876
PMID:7325796
Abstract

The endogenous N-quota in the chyme of the small intestines after providing modified proteins was determined with re-entrant cannulae at the end of the ileum in investigations with pigs (35 to 50 kg live weight) with the isotope dilution method. The N-pool of the test animals was labelled with 15N-glycine and 15N-leucine. The TCA-soluble fraction of the blood plasma served as criterion for the labelling level of the N-pool. The results showed a direct connection between N-intake and endogenous N in the chyme of the small intestines. With regard to N-intake, the dependence of endogenous N in the chyme of the small intestines on feed protein could be observed.

摘要

在对体重35至50千克的猪进行的研究中,采用同位素稀释法,通过回肠末端的再入式套管测定提供改良蛋白质后小肠食糜中的内源性氮配额。用15N-甘氨酸和15N-亮氨酸标记试验动物的氮库。血浆中三氯乙酸可溶性部分作为氮库标记水平的标准。结果表明,氮摄入量与小肠食糜中的内源性氮之间存在直接联系。就氮摄入量而言,可以观察到小肠食糜中的内源性氮对饲料蛋白质的依赖性。

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Evaluation of the 15N-isotope dilution technique for determining the recovery of endogenous protein in ileal digestion of pigs: effect of dilution in the precursor pool for endogenous nitrogen secretion.评估15N同位素稀释技术用于测定猪回肠消化中内源蛋白质回收率:内源性氮分泌前体池稀释的影响
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Evaluation of the 15N-isotope dilution technique for determining the recovery of endogenous protein in ileal digesta of pigs: effect of the pattern of blood sampling, precursor pools, and isotope dilution technique.评估¹⁵N同位素稀释技术用于测定猪回肠食糜中内源蛋白质回收率:采血模式、前体池和同位素稀释技术的影响
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引用本文的文献

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Determination of ileal endogenous nitrogen losses and true ileal nitrogen digestibility during non-steady-state conditions of the N-isotope dilution technique.采用氮稳定同位素稀释技术的非稳定状态条件下,确定回肠内源性氮损失和回肠真氮消化率。
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