不同哺乳动物肺中酰基辅酶A：溶血磷脂酰胆碱酰基转移酶和溶血磷脂酰胆碱：溶血磷脂酰胆碱转酰基酶活性的比较。

Comparison of ACYL-CoA : lysophosphatidylcholine acyltransferase and lysophosphatidylcholine : lysophosphatidylcholine transacylase activity in various mammalian lungs.

作者信息

Van Heusden G P, Van den Bosch H

出版信息

Biochim Biophys Acta. 1981 Dec 23;666(3):508-11. doi: 10.1016/0005-2760(81)90313-1.

Abstract

Lung disaturated phosphatidylcholine is thought to by synthesized, at least partially, by remodelling of unsaturated phosphatidylcholines. In view of the putative role of acyl-CoA : lysophosphatidylcholine acyltransferase (EC 2.3.1.23) and lysophosphatidylcholine : lysophosphatidylcholine transacylase in this remodelling process the activity of these enzymes in the lung of various mammals was investigated. The acyl-CoA-dependent acylation pathway was easily detected in the microsomal fractions of all animals investigated. By contrast, the enzyme catalyzing a transacylation between two molecules of lysophosphatidylcholine was only present in the 100 000 X g supernatants of rat, rabbit and mouse lung. It appeared to be undetectable in these fractions from guinea pig, bovine, pig, horse and sheep lung. These results provide and additional argument for the idea that the transacylation does not contribute to the synthesis of dipalmitoyl phosphatidylcholine in vivo.

摘要

肺中饱和磷脂酰胆碱被认为至少部分是通过不饱和磷脂酰胆碱的重塑来合成的。鉴于酰基辅酶A：溶血磷脂酰胆碱酰基转移酶（EC 2.3.1.23）和溶血磷脂酰胆碱：溶血磷脂酰胆碱转酰基酶在这一重塑过程中的假定作用，对这些酶在各种哺乳动物肺中的活性进行了研究。在所研究的所有动物的微粒体部分中，很容易检测到酰基辅酶A依赖性酰化途径。相比之下，催化两个溶血磷脂酰胆碱分子之间转酰基作用的酶仅存在于大鼠、兔子和小鼠肺的100 000×g上清液中。在豚鼠、牛、猪、马和羊肺的这些部分中似乎检测不到。这些结果为转酰基作用在体内对二棕榈酰磷脂酰胆碱合成无贡献这一观点提供了额外的论据。