Osipova S N, Mezhevikin V V, Gitel'zon I I
Biull Eksp Biol Med. 1981 Dec;92(12):688-9.
A scheme of preparing partly purified erythropoietin from rabbit blood plasma is offered. The factor of purification is about 25000. Erythropoietin was purified by ion exchange chromatography on DE-32 cellulose, affinity chromatography on phytohemagglutinin-P and by gel filtration through Ultragel ACA-44. The specific activity of erythropoietin thus obtained was about 75-100 units per mg protein. The preparation may be used for exploring the regulation and differentiation of red blood cells and as an initial material for obtaining high-purified erythropoietin..
提出了一种从兔血浆中制备部分纯化促红细胞生成素的方案。纯化因子约为25000。促红细胞生成素通过在DE-32纤维素上进行离子交换色谱、在植物血凝素-P上进行亲和色谱以及通过Ultragel ACA-44凝胶过滤进行纯化。由此获得的促红细胞生成素的比活性约为每毫克蛋白质75 - 100单位。该制剂可用于探索红细胞的调节和分化，并作为获得高纯度促红细胞生成素的起始材料。
