Familiari G, Correr S, Motta P M
Bull Assoc Anat (Nancy). 1981 Mar;65(188):83-92.
Submicroscopic alterations occurring in mouse spermatozoa during their stay in the female reproductive tract as revealed by scanning and transmission electron microscopy using ruthenium red as marker for glycosaminoglycans are described in the present investigation. The results show that the surface of the plasma membrane covering the acrosomal region of the spermatozoa found in the uterus at different intervals of time after copulation (between 10 minutes up to 20 hours), was provided with an increasing number of granules and small vesicles. Some of the surface structures appear gradually leading to perforation of discrete areas of plasma membranes in regions corresponding to the acrosomal region covering the head of spermatozoa. Also the use of ruthenium red shows a significant decrease of glycosaminoglycans and their sialic acid moieties that specifically precede further vesiculation and fenestration (perforation) of the plasma membrane covering the same acrosomal regions.
本研究描述了使用钌红作为糖胺聚糖标记物，通过扫描电子显微镜和透射电子显微镜观察到的小鼠精子在雌性生殖道停留期间发生的亚微观变化。结果表明，在交配后不同时间间隔（10分钟至20小时）在子宫中发现的精子顶体区域覆盖的质膜表面，有越来越多的颗粒和小泡。一些表面结构逐渐出现，导致在与覆盖精子头部的顶体区域相对应的区域，质膜的离散区域出现穿孔。同样，使用钌红显示，在覆盖相同顶体区域的质膜进一步形成小泡和窗孔（穿孔）之前，糖胺聚糖及其唾液酸部分显著减少。
