Creazzo T L, Sohal G S
Dev Neurosci. 1981;4(5):329-36. doi: 10.1159/000112772.
Effects of beta-bungarotoxin (beta-BTX) with enzymatically active and inactive phospholipase on the developing trochlear motor neurons and the superior oblique muscle of the duck embryo were examined. beta-BTX produced massive and widespread degeneration of neurons and skeletal muscle. When embryos were treated with beta-BTX lacking phospholipase activity the motor neurons and the skeletal muscle appeared essentially normal both at the light and the electron microscope level. These observations suggest that the toxic effects of the beta-BTX may be attributed to the phospholipase component. In the absence of phospholipase activity the toxin retained the ability to block neuromuscular transmission and when applied during the period of normal cell death it increased the number of trochlear motor neurons by sparing them from embryonic cell death.
研究了具有酶活性和无活性磷脂酶的β-银环蛇毒素（β-BTX）对鸭胚发育中的滑车运动神经元和上斜肌的影响。β-BTX导致神经元和骨骼肌大量广泛的退化。当用缺乏磷脂酶活性的β-BTX处理胚胎时，在光学显微镜和电子显微镜水平下，运动神经元和骨骼肌基本正常。这些观察结果表明，β-BTX的毒性作用可能归因于磷脂酶成分。在没有磷脂酶活性的情况下，毒素保留了阻断神经肌肉传递的能力，并且在正常细胞死亡期间应用时，它通过使滑车运动神经元免于胚胎细胞死亡而增加了其数量。
