Vaughn K C, Duke S O
Histochemistry. 1981 Dec;73(3):363-9. doi: 10.1007/BF00495650.
Tetramethylbenzidene, an artificial donor of electrons to the photosystem (PS) II reaction center, is oxidized to an osmiophilic polymer that allows for the localization of the donor site of PS II. Mesophyll chloroplasts of Sorghum bicolor (L.) Moench. contain 0.1-0.2 micron deposits only in the grana stacks and on the lumen side of the thylakoid. Agranal mature bundle sheath plastids (known to be devoid of PS II activity) show little deposition and the products appear randomly distributed along the lamellae. The cytochemical localization of PS II donor sites on the lumen side is consistent with flash photolysis and diaminobenzenesulfonic acid inactivation studies as well as the chemiosmotic theory for the generation of a proton gradient.
四甲基联苯胺是光系统（PS）II反应中心的人工电子供体，它被氧化成亲锇聚合物，从而可以定位PS II的供体部位。双色高粱（L.）Moench的叶肉叶绿体仅在基粒垛和类囊体的腔面含有0.1 - 0.2微米的沉积物。无基粒的成熟维管束鞘质体（已知缺乏PS II活性）沉积物很少，产物沿片层随机分布。PS II供体部位在腔面的细胞化学定位与闪光光解和二氨基苯磺酸失活研究以及质子梯度产生的化学渗透理论一致。
