Miller M S, Galligan J J, Burks T F
J Pharmacol Methods. 1981 Nov;6(3):211-7. doi: 10.1016/0160-5402(81)90110-8.
A new method for quantifying intestinal transit was evaluated by comparison with two other popular techniques. The distribution of radiochromium (51Cr) throughout the small intestine of rats previously treated with saline (1.0 ml/kg s.c.), capsaicin (10 mg/kg s.c.), hexamethonium (20 mg/kg i.p.), D-ala2-met-enkephalinamide (1.0 microgram i.c.v.), or neostigmine (0.1 mg/kg i.p.) was quantified by (1) measuring the most distal intestinal segment reached by chromium, (2) calculating the slope produced by linear regression analysis on cumulative percent chromium that had passed through each segment, and (3) determining the geometric center of the distribution of chromium throughout the small intestine. It was concluded that the geometric center methods for quantifying intestinal transit provides the most sensitive and reliable measure of intestinal transit. Less sensitive techniques often fail to detect important effects of drugs on intestinal transit.
通过与其他两种常用技术进行比较，评估了一种定量肠道转运的新方法。通过以下方式对放射性铬（51Cr）在先前经生理盐水（1.0 ml/kg皮下注射）、辣椒素（10 mg/kg皮下注射）、六甲铵（20 mg/kg腹腔注射）、D-丙氨酸2-甲硫氨酸脑啡肽酰胺（1.0微克脑室内注射）或新斯的明（0.1 mg/kg腹腔注射）处理的大鼠小肠中的分布进行定量：（1）测量铬到达的最远端肠段；（2）通过对穿过每个肠段的累积铬百分比进行线性回归分析计算斜率；（3）确定整个小肠中铬分布的几何中心。得出的结论是，用于定量肠道转运的几何中心法提供了最敏感和可靠的肠道转运测量方法。不太敏感的技术常常无法检测到药物对肠道转运的重要影响。
