Neiderhiser D H
Lipids. 1981 Dec;16(12):930-3. doi: 10.1007/BF02535000.
The formation of cholesteryl ester from cholesterol and acyl CoA catalyzed by the enzyme acyl CoA:cholesterol acyltransferase (EC 2.3.1.26) was studied in guinea pig gallbladder mucosa homogenate and the subcellular fractions. The enzymatic activity was enriched in the microsomal fraction. Highest activity was observed with palmitoyl, stearoyl or oleoyl CoA as substrate. Lowest activity was observed with linoleoyl CoA. These data elucidate one mechanism for the formation of cholesteryl ester from cholesterol by the gallbladder wall.
在豚鼠胆囊黏膜匀浆和亚细胞组分中，研究了由酰基辅酶A：胆固醇酰基转移酶（EC 2.3.1.26）催化胆固醇与酰基辅酶A形成胆固醇酯的过程。酶活性在微粒体组分中富集。以棕榈酰辅酶A、硬脂酰辅酶A或油酰辅酶A作为底物时观察到最高活性。以亚油酰辅酶A作为底物时观察到最低活性。这些数据阐明了胆囊壁由胆固醇形成胆固醇酯的一种机制。
