Gaĭtan V I, Vorob'eva L I
Mikrobiologiia. 1981 Nov-Dec;50(6):949-54.
During batch cultivation of Propionibacterium shermanii in a medium with glucose, ATP content in the cells (nmoles per 1 mg of dry biomass) increased up to the middle of the log phase, and decreased abruptly by the end of the log phase. When the culture was grown in a medium with lactate, the maximal level of ATP (3 nmoles/mg) was lower than in a medium with glucose (5 nmoles/mg). Nitrogen starvation of the culture was accompanied with a decrease in the level of ATP. If ammonium sulphate was added to such a culture, first the level of ATP increased and then the growth began. Addition of ammonium sulphate, after 72 h, to the nitrogen--deficient culture, the original density of whose cell suspension was over 0.25 mg/ml, did not induce the growth, although the level of ATP also increased.
在含有葡萄糖的培养基中分批培养谢氏丙酸杆菌时,细胞中的ATP含量(每1毫克干生物量中的纳摩尔数)在对数生长期中期之前升高,在对数生长期结束时突然下降。当培养物在含有乳酸的培养基中生长时,ATP的最大水平(3纳摩尔/毫克)低于在含有葡萄糖的培养基中(5纳摩尔/毫克)。培养物的氮饥饿伴随着ATP水平的降低。如果向这样的培养物中添加硫酸铵,首先ATP水平会升高,然后生长开始。在72小时后向细胞悬浮液原始密度超过0.25毫克/毫升的缺氮培养物中添加硫酸铵,虽然ATP水平也升高了,但并未诱导生长。