Daikoku S, Miyata K, Kinutani M
Neuroendocrinology. 1978;27(3-4):148-59. doi: 10.1159/000122807.
The development of gonadotrophs in newborn Sprague-Dawley rats subjected to a hypophysial stalk section (SS) by an electrical cauterization of the infundibulum of the hypothalamus was examined throughout the first 12 postnatal days. The electrical cauterization of the infundibulum was performed during 24 to 30 h after birth, and the pituitaries were studied by immunohistochemical procedures 11 days later. In several animals orchiectomy or an administration of testosterone propionate (TP) was performed simultaneously with the cauterization. The SS animals showed remarkable retardation in body growth and in pituitary development. In males the development of gonadotrophs was strongly suppressed in number and in size after the cauterization, whereas in females the suppression was less prominent. Orchiectomy promoted the development of gonadotrophs in the SS males, while TP administration suppressed it in the SS females. These findings indicate that there is sexual difference in the neonatal development of gonadotrophs even in the pituitary isolated from the hypothalamus and that testosterone inhibits the development of gonadotrophs at the level of the hypophysis during the neonatal period in rats.
通过对新生斯普拉格-道利大鼠下丘脑漏斗部进行电灼造成垂体柄切断（SS），在出生后的前12天内对促性腺激素细胞的发育进行了研究。在出生后24至30小时内对漏斗部进行电灼，11天后通过免疫组织化学方法研究垂体。在几只动物中，睾丸切除术或丙酸睾酮（TP）给药与电灼同时进行。SS动物在身体生长和垂体发育方面表现出明显迟缓。在雄性中，电灼后促性腺激素细胞的数量和大小发育受到强烈抑制，而在雌性中，这种抑制不太明显。睾丸切除术促进了SS雄性中促性腺激素细胞的发育，而TP给药则抑制了SS雌性中促性腺激素细胞的发育。这些发现表明，即使在与下丘脑分离的垂体中，促性腺激素细胞的新生发育也存在性别差异，并且睾酮在大鼠新生儿期在垂体水平抑制促性腺激素细胞的发育。
