Kennedy P S, Ware J, Horak J K, Solis R
Thromb Haemost. 1981 Dec 23;46(4):725-30.
Factors affecting the size distribution of platelet aggregates formed in whole blood were measured using an electronic particle counter. Lysis of red cells after dilution of blood for analysis allows detection of smaller aggregates without altering aggregate measurement. Differences in platelet response to increasing concentrations of ADP are detected by an increase in the mean size of platelet aggregates while the total volume of aggregates varies linearly with the volume of available platelets (r = .99). The mean aggregate size varies directly with the total volume of aggregates formed (r = .99). Mean aggregate size is inversely related to the packed red cell volume. As the reactivity of platelets is reduced by incubation with aggregation inhibitors at increasing concentrations, the mean size of aggregates becomes smaller, followed by a decrease in the total volume of aggregates. The total volume and mean size of aggregates formed after incubation with collagen and epinephrine, but not ADP, increases with time after venepuncture.
使用电子粒子计数器测量影响全血中形成的血小板聚集体大小分布的因素。在稀释血液进行分析后裂解红细胞，可检测到较小的聚集体，同时不改变聚集体的测量结果。通过血小板聚集体平均大小的增加来检测血小板对递增浓度ADP反应的差异，而聚集体的总体积与可用血小板的体积呈线性变化（r = 0.99）。聚集体的平均大小与形成的聚集体总体积直接相关（r = 0.99）。平均聚集体大小与红细胞压积呈负相关。随着血小板与浓度递增的聚集抑制剂孵育，其反应性降低，聚集体的平均大小变小，随后聚集体的总体积减少。与胶原蛋白和肾上腺素（而非ADP）孵育后形成的聚集体的总体积和平均大小，在静脉穿刺后的时间里会增加。
