烷基化剂诱导C3H/10T1/2 CL8细胞发生形态学转变。
Morphological transformation of C3H/10T1/2 CL8 cells by alkylating agents.
作者信息
Oshiro Y, Balwierz P S, Molinary S V
出版信息
Toxicol Lett. 1981 Dec;9(4):301-6. doi: 10.1016/0378-4274(81)90001-1.
The standard method of the C3H/10T1/2 cell transformation assay cannot adequately detect alkylating agents. A modification of the standard procedure as described by Bertram and Heidelberger using a large number of synchronized cells and high levels of toxicity was evaluated for transformation using several alkylating agents. By using this method, N-methyl-N' -nitro-N-nitrosoguanidine (MNNG), beta-propiolactone (BPL), methyl methanesulfonate (MMS), methylnitrosourea (MNU) and 1,3-propane sultone (PS) transformed these cells. However, methyl iodide (MI) failed to induce any transformed foci.
C3H/10T1/2细胞转化试验的标准方法无法充分检测烷基化剂。采用Bertram和Heidelberger描述的标准程序的一种改进方法，使用大量同步化细胞和高毒性水平，对几种烷基化剂进行转化评估。通过使用这种方法，N-甲基-N'-硝基-N-亚硝基胍（MNNG）、β-丙内酯（BPL）、甲磺酸甲酯（MMS）、甲基亚硝基脲（MNU）和1,3-丙烷磺酸内酯（PS）使这些细胞发生了转化。然而，甲基碘（MI）未能诱导出任何转化灶。
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