Arancia G, Gripari E, Rosati F, Trovalusci P, Meli D
Eur J Cell Biol. 1981 Oct;25(2):272-7.
A freeze-fracture study has been carried out on human peripheral blood lymphocytes (hPBL) from healthy donors. Lymphocytes were frozen either from 37 degrees C or 4 degrees C. Quantitative analysis performed on density and size of intramembranous particles (IMPs) present on both fracture faces of the plasma membrane has revealed: a) a difference in size between IMPs on the external face (EF) and those on the protoplasmic face (PF); b) a remarkable influence of temperature either on size or density of the IMPs; c) the existence at 4 degrees C of two lymphocyte populations differing in IMP density.
对来自健康供体的人外周血淋巴细胞(hPBL)进行了冷冻蚀刻研究。淋巴细胞分别从37℃或4℃冷冻。对质膜两个断裂面上存在的膜内颗粒(IMP)的密度和大小进行的定量分析表明:a)外表面(EF)上的IMP与原生质表面(PF)上的IMP在大小上存在差异;b)温度对IMP的大小或密度有显著影响;c)在4℃时存在两个IMP密度不同的淋巴细胞群体。