Hamana K
J Biochem. 1981 Dec;90(6):1591-6. doi: 10.1093/oxfordjournals.jbchem.a133633.
Five histone molecular species, H1, H2A, H2B, H3, and H4, of calf thymus were subjected to gel electrophoresis and gel chromatography in the presence of a cationic surfactant, cetyltrimethylammonium bromide (CTAB), and their behavior was compared with that in the presence of an anionic surfactant, sodium dodecyl sulfate (SDS). In the presence of CTAB, the retardation coefficient (KR) and distribution coefficient (Kd) values calculated for the five histone species were linearly related to their molecular weights, without showing abnormality of the type found in the presence of SDS. It was concluded that CTAB-gel electrophoresis is useful for the analysis of histones.
对小牛胸腺的五种组蛋白分子种类H1、H2A、H2B、H3和H4，在阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）存在的情况下进行凝胶电泳和凝胶色谱分析，并将它们的行为与在阴离子表面活性剂十二烷基硫酸钠（SDS）存在时的行为进行比较。在CTAB存在的情况下，计算出的这五种组蛋白种类的阻滞系数（KR）和分配系数（Kd）值与它们的分子量呈线性关系，未表现出在SDS存在时所发现的那种异常情况。得出的结论是，CTAB-凝胶电泳可用于组蛋白的分析。
