Akin D T, Shapira R, Kinkade J M
Anal Biochem. 1985 Feb 15;145(1):170-6. doi: 10.1016/0003-2697(85)90343-4.
A novel cetyltrimethylammonium bromide-polyacrylamide gel electrophoresis system which is useful for the separation of native forms of proteins consistent with their molecular weights is reported here. Many proteins examined in this system demonstrated the same association patterns which have been shown by other techniques to exist under nondenaturing conditions. In addition, biological activity could be assayed directly in the gel after electrophoresis. Based on the peculiar characteristics of cetyltrimethylammonium bromide, a possible explanation which may account for the behavior of proteins in this system is presented.
本文报道了一种新型的十六烷基三甲基溴化铵-聚丙烯酰胺凝胶电泳系统，该系统可用于分离与蛋白质分子量相符的天然形式。在该系统中检测的许多蛋白质表现出与其他技术在非变性条件下所显示的相同缔合模式。此外，电泳后可直接在凝胶中测定生物活性。基于十六烷基三甲基溴化铵的特殊特性，提出了一个可能解释该系统中蛋白质行为的原因。
