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女性、大鼠和家兔输卵管的“体外”培养。初步结果(作者译)

["In vitro" culture of the oviduct of women, rats and rabbits. Preliminary results (author's transl)].

作者信息

Menezo Y, Bremond A, Khatchadourian C, Orgiazzi A, Rudigoz R

出版信息

J Gynecol Obstet Biol Reprod (Paris). 1981;10(7):651-4.

PMID:7334190
Abstract

A continuous flow perfusion system which has been improved for the culture of large pieces of tissue, e.g. epididymis and ovarian follicles was tested for the entire oviduct in women, rabbits and rats. The histological properties of the organ were maintained after a day or two of culture; but only in the presence of oestrogens. Progesterone seems to induce a weakness of epithelial cell attachment and these become scattered in the lumen. In women the cultures seemed to be more or less permanently contamined, probably due to commensals, because this problem did not occur with rat or rabbit oviducts.

摘要

一种经过改进用于大块组织(如附睾和卵巢卵泡)培养的连续流动灌注系统,在女性、兔子和大鼠的整个输卵管上进行了测试。培养一两天后,该器官的组织学特性得以维持,但前提是存在雌激素。孕酮似乎会导致上皮细胞附着减弱,这些细胞会分散在管腔中。在女性中,培养物似乎或多或少会受到永久性污染,可能是由于共生菌,因为大鼠或兔子的输卵管并未出现这个问题。

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