Gumpel-Pinot M
J Embryol Exp Morphol. 1981 Oct;65:73-87.
Limb ectoderm induces cartilage differentiation in mesoderm from chick embryo limb buds, Transfilter cultures have shown that this interactions requires 'contact' conditions and cannot take place at a distance. In vivo, a basement membrane is always present between ectoderm and mesoderm. The present paper demonstrates that the relationship between ectoderm and mesoderm is similar in vivo and in transfilter cultures. In culture conditions, the filter appears to be infiltrated by mesodermal cell outgrowths which form a continuous mesodermal cover on the filter. A basement membrane is always present between the mat of mesodermal cell processes and the ectoderm. Mesodermal cell processes are able to cross the Nuclepore filters (pore size 0.6-0.8 micrometer) within 15 min. After 2 h in culture, the surface of the filter opposite to the mesodermal explant is completely covered with mesodermal outgrowths. The extracellular material accumulating at the ectoderm-mesoderm interface appears to be mainly of mesodermal origin.
肢体外胚层可诱导鸡胚肢体芽中胚层的软骨分化。透膜培养表明，这种相互作用需要“接触”条件，无法在远距离发生。在体内，外胚层和中胚层之间始终存在基底膜。本文证明，外胚层和中胚层之间的关系在体内和透膜培养中是相似的。在培养条件下，滤膜似乎被中胚层细胞生长物浸润，这些生长物在滤膜上形成连续的中胚层覆盖层。中胚层细胞生长物与外胚层之间始终存在基底膜。中胚层细胞生长物能够在15分钟内穿过核孔滤膜（孔径0.6 - 0.8微米）。培养2小时后，滤膜与中胚层外植体相对的表面完全被中胚层生长物覆盖。在外胚层 - 中胚层界面积累的细胞外物质似乎主要起源于中胚层。
