Garg H S, Awasthi Y C, Srivastava S K
J Neurosci Res. 1981;6(6):771-83. doi: 10.1002/jnr.490060611.
A method has been developed for the complete extraction and fractionation of the autofluorescent lipopigments present in the neuronal tissue of Batten-Spielmeyer-Vogt (BSV) patient and normal subject, respectively. The fluorescent lipopigments that were about three-fold higher in BSV patient's brain as compared with normal subjects were separated into at least 7 fluorometrically and chromatographically distinct fractions by thin layer chromatography (TLC). Although the fluorescent lipopigments of the brain tissue of BSV patient and normal subject showed identical pattern of separation on TLC, the flourescence spectra of the individual lipopigment fractions from BSV patient was different from those of the corresponding fractions from the normal subject. The ultraviolet spectral studies with individual fluorescent lipopigment fractions indicated the presence of highly unsaturated conjugated chromophore.
已经开发出一种方法，分别用于从巴顿-施皮勒迈尔-沃格特（BSV）患者和正常受试者的神经元组织中完全提取和分离自发荧光脂色素。与正常受试者相比，BSV患者大脑中荧光脂色素含量高出约三倍，通过薄层色谱法（TLC）将其分离为至少7个在荧光和色谱上不同的组分。尽管BSV患者和正常受试者脑组织的荧光脂色素在TLC上显示出相同的分离模式，但BSV患者单个脂色素组分的荧光光谱与正常受试者相应组分的荧光光谱不同。对单个荧光脂色素组分的紫外光谱研究表明存在高度不饱和的共轭发色团。
