[将源自小鼠脾脏的非组蛋白染色质蛋白显微注射到L细胞中]
[Microinjection of non-histone chromatin protein derived from mouse spleen into L cells].
作者信息
Karavanov A A, Cherinkov V G, Afanas'ev B N, Liashko V N
出版信息
Mol Biol (Mosk). 1981 Jan-Feb;15(1):234-42.
PMID:7335076
Abstract
Non-histone protein selectively released from mouse spleen nuclei by mild hydrolysis with micrococcal nuclease or DNase I was introduced into L cells by the microinjection method. It is shown that this protein selectively accumulates in cell nuclei. Analysis of the mitotic cells after microinjection of 125I labeled protein demonstrated its location in chromosomes of L cells.
摘要
通过用微球菌核酸酶或脱氧核糖核酸酶I进行温和水解从小鼠脾细胞核中选择性释放的非组蛋白，采用显微注射法被导入L细胞。结果表明，这种蛋白质选择性地在细胞核中积累。对显微注射125I标记蛋白质后的有丝分裂细胞进行分析，证实了其在L细胞染色体中的定位。
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