Stoffyn A, Stoffyn P, Farooq M, Snyder D S, Norton W T
Neurochem Res. 1981 Oct;6(10):1149-57. doi: 10.1007/BF00964419.
Total homogenates of astrocytes, neuronal perikarya, and oligodendroglia isolated from rat brain exhibited sialosyltransferase activity in the biosynthesis in vitro of GM3 from lac-cer and CMP-NeuNAc. This activity was highest in neuronal perikarya. In contrast, only oligodendroglia were found to be capable of catalyzing the transfer of NeuNAc from CMP-NeuNAc to gal-cer to form GM4. This activity was about three fold higher in oligodendroglia from 10-day-old rat brain than in those from 60-day-old animals; an intermediate activity was observed in oligodendroglia from 20-day-old rats. Purified preparations of myelin from 14-day-old rat cerebrum, cerebellum, and spinal cord did not show activity in the synthesis of either GM3 or GM4.
从大鼠脑中分离出的星形胶质细胞、神经元胞体和少突胶质细胞的总匀浆在体外由乳糖神经酰胺和CMP-唾液酸合成GM3的生物合成过程中表现出唾液酸转移酶活性。这种活性在神经元胞体中最高。相比之下，仅发现少突胶质细胞能够催化将CMP-唾液酸中的唾液酸转移至半乳糖神经酰胺以形成GM4。来自10日龄大鼠脑的少突胶质细胞中的这种活性比来自60日龄动物的少突胶质细胞中的活性高约三倍；在20日龄大鼠的少突胶质细胞中观察到中等活性。来自14日龄大鼠大脑、小脑和脊髓的纯化髓鞘制剂在GM3或GM4的合成中均未表现出活性。
