Schmitz N, Djibey I, Kretschmer V, Mahn I, Mueller-Eckhardt C
Vox Sang. 1981;41(4):224-30. doi: 10.1111/j.1423-0410.1981.tb01040.x.
To evaluate the applicability of a radioactive 125I-anti-IgG test (RIAT) for the detection of small amounts of IgG antibodies on red blood cells (RBC) of patients with autoimmune hemolytic anemia of warm type (AIHA), RBC of 125 patients were studied (AIHA, n = 53; Coombs'-negative AIHA, n = 6; chronic cold agglutinin disease, n = 7; non-immune anemias, n = 59). It was found that the RBC of all cases (33/33) with a positive direct IgG antiglobulin test (DAT-IgG), but also 13 out of 20 patients with a negative DAT-IgG, but detectable complement (C3/C4), and 4 out of 6 cases with Coombs'-negative AIHA gave positive results in the direct RIAT. RBC-associated IgG was higher in the DAT-IgG positive group (n = 33; -x = 8.1%) than in the DAT-IgG negative group (n = 26; -x3.4%). There was no correlation between hypergammaglobulinemia and RBC-associated IgG. The sensitivity of the indirect RIAT was not remarkable better as compared to the indirect antiglobulin test. The RIAT is valuable in the serology of borderline cases of AIHA.
为评估放射性¹²⁵I-抗IgG试验（RIAT）在检测温抗体型自身免疫性溶血性贫血（AIHA）患者红细胞（RBC）上少量IgG抗体中的适用性，对125例患者的RBC进行了研究（AIHA，n = 53；抗人球蛋白试验阴性的AIHA，n = 6；慢性冷凝集素病，n = 7；非免疫性贫血，n = 59）。结果发现，直接IgG抗球蛋白试验（DAT-IgG）阳性的所有病例（33/33）的RBC，以及DAT-IgG阴性但可检测到补体（C3/C4）的20例患者中的13例，还有6例抗人球蛋白试验阴性的AIHA病例中的4例，直接RIAT结果均为阳性。DAT-IgG阳性组（n = 33；-x = 8.1%）的RBC相关IgG高于DAT-IgG阴性组（n = 26；-x = 3.4%）。高球蛋白血症与RBC相关IgG之间无相关性。间接RIAT的敏感性与间接抗球蛋白试验相比并无明显提高。RIAT在AIHA临界病例的血清学检查中具有重要价值。
