锰对生长中大鼠脑儿茶酚胺合成的影响。

Effect of manganese on synthesis of brain catecholamines in growing rats.

作者信息

Chandra S V, Shukla G S

出版信息

Acta Pharmacol Toxicol (Copenh). 1981 Apr;48(4):349-54. doi: 10.1111/j.1600-0773.1981.tb01631.x.

DOI:10.1111/j.1600-0773.1981.tb01631.x

Abstract

The effect of short-term exposure of manganese on the synthesis of brain catecholamines in growing rats has been investigated by using two methods, 1) measuring the amount of conversion of 3,5-3H-tyrosine to 3H-labelled dopamine and norepinephrine, and 2) measuring the rate of decline of endogenous dopamine and norepinephrine following inhibition of their synthesis with alpha-methyl-p-tyrosine. The magnitude of conversion of 3,5-3H-tyrosine to 3H-dopamine and 3H-norepinephrine in the brain at 60 min after the administration of labelled amino acid to the manganese treated rats (1 mg MnCl2.4H2O/ml of water for 30 days) was significantly greater than observed in control rats. Manganese also increased turnover of dopamine and norepinephrine to a greater extent in the brains of treated animals.

摘要

通过两种方法研究了短期接触锰对生长中大鼠脑儿茶酚胺合成的影响

1）测量3,5-³H-酪氨酸向³H标记的多巴胺和去甲肾上腺素的转化量；2）在用α-甲基-p-酪氨酸抑制其合成后，测量内源性多巴胺和去甲肾上腺素的下降速率。给锰处理的大鼠（1mg MnCl₂·4H₂O/毫升水，持续30天）注射标记氨基酸60分钟后，其脑中3,5-³H-酪氨酸向³H-多巴胺和³H-去甲肾上腺素的转化量显著高于对照大鼠。锰还使处理动物脑中多巴胺和去甲肾上腺素的周转率有更大程度的增加。