Langford G M
Biosystems. 1981;14(3-4):247-59. doi: 10.1016/0303-2647(81)90032-0.
Shark and beef brain tubulins were prepared by a common temperature-dependent centrifugation procedure and compared on the basis of assembly properties. The data show that: (a) the conditions for spontaneous assembly without microtubule associated proteins; (b) initial rates of assembly between 20 degrees C and 37 degree C; and (c) types of polymers formed in the presence of Ca2+ are different for the two types of tubulins. The number of protofilaments, on the other hand, is the same for microtubules reconstituted from the two species of tubulins. These data are interpreted to suggest that tubulins from different species have unique properties, but tubulin sites affecting the structure of microtubules have been highly conserved.
鲨鱼和牛脑微管蛋白通过一种常见的温度依赖性离心程序制备，并根据组装特性进行比较。数据表明：（a）无微管相关蛋白时自发组装的条件；（b）20摄氏度至37摄氏度之间的初始组装速率；（c）两种类型的微管蛋白在Ca2+存在下形成的聚合物类型不同。另一方面，由两种微管蛋白重构的微管原纤维数量相同。这些数据被解释为表明不同物种的微管蛋白具有独特的特性，但影响微管结构的微管蛋白位点高度保守。
