用2-硝基-4-叠氮苯甲酰-苯丙氨酰-tRNA对不同脊椎动物肝脏核糖体进行亲和标记
Affinity labeling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-phe-tRNA.
作者信息
Stahl J, Böhm H, Voderberg M
出版信息
Acta Biol Med Ger. 1981;40(9):1101-4.
PMID:7340334
Abstract
Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-Nitro-4-azidobenzoyl-Phe-tRNA in affinity labeling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates.
摘要
在亲和标记实验中发现，来自鳟鱼、母鸡和大鼠肝脏的核糖体蛋白L10是2-硝基-4-叠氮苯甲酰苯丙氨酰-tRNA的主要作用靶点。因此，尽管该蛋白的电泳迁移率略有不同，但它似乎参与了各种脊椎动物核糖体中肽基转移酶中心的组成。
相似文献
1
Affinity labeling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-phe-tRNA.用2-硝基-4-叠氮苯甲酰-苯丙氨酰-tRNA对不同脊椎动物肝脏核糖体进行亲和标记
Acta Biol Med Ger. 1981;40(9):1101-4.
2
Photoaffinity labeling of rat liver ribosomes by N-(2-nitro-4-azidobenzoyl)puromycin.用N-(2-硝基-4-叠氮苯甲酰)嘌呤霉素对大鼠肝脏核糖体进行光亲和标记。
Acta Biol Med Ger. 1979;38(10):1447-52.
3
Present conceptions on the peptidyltransferase centre of animal ribosomes.关于动物核糖体肽基转移酶中心的当前概念。
Biomed Biochim Acta. 1983;42(1):47-55.
4
[Modifications of ribosomes from rat liver with alkylating derivatives of tRNA].[用tRNA的烷基化衍生物对大鼠肝脏核糖体进行的修饰]
Mol Biol (Mosk). 1976 May-Jun;10(2):670-4.
5
Identification of proteins at the peptidyl-tRNA binding site of rat liver ribosomes.大鼠肝脏核糖体肽基 - tRNA结合位点蛋白质的鉴定
Mol Gen Genet. 1981;184(3):551-6. doi: 10.1007/BF00352539.
6
Affinity labelling of yeast ribosomal peptidyl transferase.酵母核糖体肽基转移酶的亲和标记
Mol Gen Genet. 1978 Jul 6;163(1):29-34. doi: 10.1007/BF00268961.
7
Photoaffinity labeling of Escherichia coli ribosomes with an aryl azide analogue of puromycin.用嘌呤霉素的芳基叠氮类似物对大肠杆菌核糖体进行光亲和标记。
FEBS Lett. 1978 Jun 15;90(2):203-8. doi: 10.1016/0014-5793(78)80369-x.
8
Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions.通过光亲和标记法对23S rRNA中精胺结合位点进行定位：解析精胺对核糖体50S亚基功能的贡献
Nucleic Acids Res. 2005 May 16;33(9):2792-805. doi: 10.1093/nar/gki557. Print 2005.
9
Affinity labeling of peptidyl transferase center using the 3' terminal pentanucleotide from amino acyl-tRNA.使用来自氨酰 - tRNA的3'末端五核苷酸对肽基转移酶中心进行亲和标记。
Biochem Biophys Res Commun. 1983 Jun 29;113(3):941-7. doi: 10.1016/0006-291x(83)91089-6.
10
Photoaffinity labeling of peptidyltransferase.肽基转移酶的光亲和标记
Methods Enzymol. 1988;164:361-72. doi: 10.1016/s0076-6879(88)64055-9.
Zotero 插件