Affinity labeling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-phe-tRNA.

Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-Nitro-4-azidobenzoyl-Phe-tRNA in affinity labeling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates.