Watson D L, Kennedy J W
Aust Vet J. 1981 Jul;57(7):309-13. doi: 10.1111/j.1751-0813.1981.tb05834.x.
Ewes were immunised in late pregnancy with killed Staphylococcus aureus vaccines prepared from organisms grown either under in vitro (vaccine T) or in vivo (Vaccine V) cultural conditions; other ewes were immunised with a live S. aureus vaccine and a further group remained non-vaccinated controls. The animals given either of the killed vaccines developed highest titres of agglutinating antibody in serum; there were only trivial levels of agglutinating antibody in milk from ewes in each treatment group. Ewes immunised with the live vaccine developed significantly greater levels of opsonins in serum than did those immunised with the killed vaccines or non-immunised controls. At 30 to 35 days post-partum the ewes were challenged by intramammary infusion of one million S. aureus of a strain different to the vaccination strain. In 4 of the 5 control ewes this resulted in the development of acute mastitis and a precipitous decline in milk production, whereas there was a considerable degree of resistance recorded in animals in each of the vaccinated groups. On criteria of milk production data, bacteriological status of milk and clinical signs of acute mastitis it was apparent that animals which had been immunised with the live vaccine were better protected from challenge than those immunised with either killed vaccines T or V.
在怀孕后期，用由在体外（疫苗T）或体内（疫苗V）培养条件下生长的金黄色葡萄球菌制备的灭活疫苗对母羊进行免疫；其他母羊用活的金黄色葡萄球菌疫苗进行免疫，另一组作为未接种疫苗的对照。接受任何一种灭活疫苗的动物血清中凝集抗体滴度最高；每个处理组母羊的乳汁中只有微量的凝集抗体。用活疫苗免疫的母羊血清中的调理素水平明显高于用灭活疫苗免疫的母羊或未免疫的对照。产后30至35天，通过向乳腺内注入100万个与疫苗株不同的金黄色葡萄球菌菌株对母羊进行攻毒。5只对照母羊中有4只因此发生了急性乳腺炎，产奶量急剧下降，而每个接种组的动物都表现出相当程度的抵抗力。根据产奶数据、乳汁的细菌学状况和急性乳腺炎的临床症状标准，显然用活疫苗免疫的动物比用灭活疫苗T或V免疫的动物对攻毒的保护作用更好。
