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金鱼脑垂体中间部对去离子水(无论是否缓冲)以及钙和镁补充剂的反应(作者译)

[Response of the pars intermedia of the goldfish pituitary to deionized water, buffered or not, and to calcium and magnesium supplementation (author's transl)].

作者信息

Olivereau M, Aimar C, Olivereau J M

出版信息

J Physiol (Paris). 1981 Mar;77(8):895-8.

PMID:7341768
Abstract

In the goldfish, one cell type of the pars intermedia, slightly PAS positive, is highly stimulated in deionized water (E Dés). This response is not influenced by the pH of the water (E Dés pH 5.3, E Dés buffered with imidazole pH 6.7) for 20 days. Stimulation is prevented when the E Dés is supplemented with CaCl2 (2mM), but it is not affected by the addition of MgCl2 (2 mM). When goldfish are kept in E Dés for 20 days, and subsequently in E Dés supplemented with calcium gluconate (1 mM), a granule storage occurs in the cytoplasm of the enlarged cells within 48 hours. These cells are therefore sensitive to the lack or presence of calcium in the environment. The term of calcium-sensitive (Ca-s) cells appears justified in the goldfish, as in the eel; their putative role in the control of the pigmentation is not evident.

摘要

在金鱼中,脑垂体中间部的一种细胞类型,对过碘酸雪夫反应(PAS)呈弱阳性,在去离子水(E Dés)中受到高度刺激。这种反应在20天内不受水的pH值影响(E Dés pH 5.3,用咪唑缓冲至pH 6.7的E Dés)。当在E Dés中添加氯化钙(2mM)时,刺激作用被阻止,但添加氯化镁(2 mM)对其没有影响。当金鱼在E Dés中饲养20天,随后置于添加葡萄糖酸钙(1 mM)的E Dés中时,在48小时内,扩大细胞的细胞质中会出现颗粒储存。因此,这些细胞对环境中钙的缺乏或存在敏感。与鳗鱼一样,“钙敏感(Ca-s)细胞”这一术语在金鱼中似乎是合理的;它们在色素沉着控制中的假定作用并不明显。

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