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[牛心脏房室系统中糖原含量、糖酵解活性及代谢产物含量(作者译)]

[Contents of glycogen, glycolytic activity and contents of metabolites in the atrioventricular system of bovine hearts (author's transl)].

作者信息

Thorn W, Nitsche D, Harms B, Müldener B, Bartels J

出版信息

Res Exp Med (Berl). 1978 Dec 27;174(1):67-78. doi: 10.1007/BF01851940.

Abstract

In the atrioventricular system (AVS) consisting of the compact node, the penetrating bundle and the branching bundles of about 250 bovine hearts there were made several studies: 1. In quickly removed and fixed specimens (distal AV-node, penetrating bundle) determination of a metabolic state with respect to glycogen, glucose, lactate, ATP, ADP, AMP, creatinephosphate, total creatine, gluc-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, dihydroxyacetonphosphate and pyruvate. 2. Determination of glycogen contents and glygolytic activity in AVS and its parts for ischemic times up to three hours. 3. The determination of metabolic contents in samples of connective tissue in atrium and ventricle of bovine hearts. The AV-nodes are poor in glycogen comparable with glycogen content of central nervous system and other ganglia. Penetrating bundles of Hiss and branching bundle belong after liver to the glycogen richest parenchyma of animal tissues. Even after ischemia of 3 h only a part of glycogen was recovered as lactate. The greater part of glycogen must be considered as a structural element of Hiss bundle and branching bundles of the ventricles.

摘要

在由致密结、穿入束和分支束组成的房室系统(AVS)中,对约250个牛心脏进行了多项研究:1. 在快速取出并固定的标本(远端房室结、穿入束)中,测定糖原、葡萄糖、乳酸、三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)、磷酸肌酸、总肌酸、6-磷酸葡萄糖、6-磷酸果糖、1,6-二磷酸果糖、磷酸二羟丙酮和丙酮酸的代谢状态。2. 测定房室系统及其各部分在长达3小时的缺血时间内的糖原含量和糖酵解活性。3. 测定牛心脏心房和心室结缔组织样本中的代谢成分。与中枢神经系统和其他神经节的糖原含量相比,房室结的糖原含量较低。希氏束的穿入束和分支束仅次于肝脏,属于动物组织中糖原最丰富的实质组织。即使在缺血3小时后,也只有一部分糖原以乳酸的形式被回收。大部分糖原必须被视为心室希氏束和分支束的结构成分。

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