Santoro S A, Cunningham L W
Coll Relat Res. 1981 Nov;1(6):517-26. doi: 10.1016/s0174-173x(81)80033-7.
Multiple, linked interactions between the platelet surface and collagen fibers have been implicated in the initiation of platelet secretion and subsequent aggregation. The formation of such multiple simultaneous interactions could give rise to high affinity adhesion of platelets to collagen even though the affinity of the individual interactions may be much weaker. This concept has been tested by measuring the adhesion of platelets to collagen under conditions which could effect the formation of multiple interactions. Adhesion is markedly diminished at 4 degrees C but not at 23 or 37 degrees C. Metabolic inhibitors such as 2-deoxyglucose and Antimycin A do not inhibit adhesion although they virtually abolish subsequent aggregation. Brief formaldehyde fixation of platelets greatly reduces adhesion. These results are consistent with the concept that the formation of multiple linked interactions between the platelet surface and collagen are important in platelet-collagen adhesion and that mobility of platelet membrane components is required for the clustering of these interactions in focussed regions on the platelet surface.
血小板表面与胶原纤维之间存在多种相互关联的相互作用，这些作用与血小板分泌的起始及随后的聚集有关。即使单个相互作用的亲和力可能较弱，但这种多个同时发生的相互作用的形成可能导致血小板与胶原的高亲和力黏附。通过在可能影响多个相互作用形成的条件下测量血小板与胶原的黏附，对这一概念进行了验证。在4℃时黏附明显减少，但在23℃或37℃时则不然。代谢抑制剂如2-脱氧葡萄糖和抗霉素A虽几乎完全消除随后的聚集，但并不抑制黏附。血小板经短暂甲醛固定后，黏附力大大降低。这些结果与以下概念一致，即血小板表面与胶原之间多个相互关联的相互作用的形成在血小板-胶原黏附中很重要，并且血小板膜成分的流动性是这些相互作用在血小板表面特定区域聚集所必需的。
