Riley V, Spackman D H
Nutr Cancer. 1981;2(3):181-92. doi: 10.1080/01635588109513679.
The endogenous asparagine influx in mice undergoing asparaginase therapy can be neutralized by asparaginase activity equivalent to less than one IU/ml of plasma, as determined with a new, highly sensitive micro assay for asparaginase. It appears feasible that an asparagine influx of this magnitude may be cleared from the blood plasma by hemodialysis employing an artificial kidney apparatus with a clearance capacity somewhat greater than that routinely employed by renal patients. Application of this method for removing asparagine or other amino acids from the blood as an approach to cancer therapy ultimately depends upon the specific amino acid influx rates of the subject and upon the ability of the dialyzer to deplete the free amino acids in the blood plasma at a sufficiently high rate to lower specific amino acid concentrations in malignant tissues, or in adjoining interstitial spaces, to therapeutic levels. Based upon the data available, hemodialysis has potential therapeutic application as a means to control the removal of various free amino acids or other metabolic substrates from blood plasma.
用一种新的高灵敏度微量测定法测定，接受天冬酰胺酶治疗的小鼠体内内源性天冬酰胺流入量可被相当于血浆中低于1国际单位/毫升的天冬酰胺酶活性中和。通过使用一种清除能力略高于肾病患者常规使用的人工肾装置进行血液透析，从血浆中清除这种数量的天冬酰胺流入量似乎是可行的。作为癌症治疗的一种方法，应用这种方法从血液中去除天冬酰胺或其他氨基酸最终取决于受试者特定氨基酸的流入速率，以及透析器以足够高的速率消耗血浆中游离氨基酸从而将恶性组织或相邻间质空间中的特定氨基酸浓度降低到治疗水平的能力。根据现有数据，血液透析作为一种控制从血浆中去除各种游离氨基酸或其他代谢底物的手段具有潜在的治疗应用价值。
