Hsueh A J, Erickson G F
Steroids. 1978 Dec;32(5):639-48. doi: 10.1016/0039-128x(78)90074-0.
The effects of glucocorticoids on the steroidogenesis of ovarian granulosa cells were investigated. Cortisol and dexamethasone inhibited the increase in aromatase activity induced by FSH in cultured rat granulosa cells. In the same cultures progesterone production was stimulated to a maximum of 167% of the control level. This differential effect of glucocorticoids on estrogen and progesterone production by the granulosa cells indicates that glucocorticoids exert specific inhibition of the induction of aromatase by FSH and do not cause a general suppression of granulosa cell activity. In contrast to their inhibition of the FSH induction of aromatase enzymes, glucocorticoids did not interfere with the activity of pre-existing aromatase enzymes. In granulosa cells containing full aromatase activity, treatment with cortisol and dexamethasone did not inhibit aromatization of androstenedione to estrogens whereas two known aromatase inhibitors (dihydrotestosterone and 4-androstene-3, 6, 17-trione) were effective. These results indicate that the glucocorticoids exert a selective inhibition of the FSH-induction of aromatase activity in rat granulosa cells by a mechanism other than directly interfering with the aromatization reaction.
研究了糖皮质激素对卵巢颗粒细胞类固醇生成的影响。皮质醇和地塞米松抑制了促卵泡激素（FSH）诱导培养的大鼠颗粒细胞中芳香化酶活性的增加。在相同培养条件下，孕酮生成被刺激至对照水平的167%。糖皮质激素对颗粒细胞雌激素和孕酮生成的这种差异作用表明，糖皮质激素对FSH诱导芳香化酶具有特异性抑制作用，而不会引起颗粒细胞活性的普遍抑制。与它们对FSH诱导芳香化酶的抑制作用相反，糖皮质激素并不干扰已存在的芳香化酶的活性。在具有完全芳香化酶活性的颗粒细胞中，用皮质醇和地塞米松处理不会抑制雄烯二酮向雌激素的芳香化作用，而两种已知的芳香化酶抑制剂（双氢睾酮和4-雄烯-3,6,17-三酮）则有效。这些结果表明，糖皮质激素通过一种并非直接干扰芳香化反应的机制，对大鼠颗粒细胞中FSH诱导的芳香化酶活性发挥选择性抑制作用。
