Fried W, Barone-Varelas J, Barone T, Helfgott M
Exp Hematol. 1980;8 Suppl 8:41-51.
Erythropoietin (Ep) in large amounts was detected in extracts of renal tissue from hypoxic rats. These extractions were performed by homogenization of kidney tissue in phosphate buffered saline, centrifugation at 3,000 g and collection of the supernate. Male kidneys contained more Ep than did females and the major portion of Ep is located in the renal cortex. Comparison of intrarenal and plasma Ep levels at various times following initiation and cessation of hypoxia appears to be a useful method for studying the kinetics of erythropoietin production and release, and also for studying feedback mechanisms that influence these functions.
在低氧大鼠的肾组织提取物中检测到大量促红细胞生成素（Ep）。这些提取物是通过将肾组织在磷酸盐缓冲盐水中匀浆、3000g离心并收集上清液来制备的。雄性肾脏中的Ep含量高于雌性，且Ep的主要部分位于肾皮质。比较低氧开始和停止后不同时间肾内和血浆中的Ep水平，似乎是研究促红细胞生成素产生和释放动力学以及影响这些功能的反馈机制的一种有用方法。
