Nassar C F, Khuri R N, Hajjar J J
Biochim Biophys Acta. 1980;595(1):121-5. doi: 10.1016/0005-2736(80)90253-9.
The influx of alanine across the serosal membrane of Testudo graeca intestinal cells with preserved epithelial orientation was examined. Our results suggest that: 1. The mechanism of alanine influx across the serosal membrane of turtle intesintal cells is a carrier-mediated process that has the characteristics of facilitated diffusion. 2. Alanine influx mechanism is independent of intra- and extra-cellular changes in Na+ and K+ concentrations, and is not altered by reversal of Na+ and K+ gradients across the serosal membrane. 3. In Na+-free media the mechanism of transport of alanine at the mucosal membrane has the same pattern of competitive inhibition by amino acids as the serosal.
对具有完整上皮方向的希腊陆龟肠道细胞浆膜上丙氨酸的流入情况进行了检测。我们的结果表明：1. 龟肠道细胞浆膜上丙氨酸的流入机制是一种载体介导的过程，具有易化扩散的特征。2. 丙氨酸流入机制独立于细胞内和细胞外Na⁺和K⁺浓度的变化，并且不会因浆膜上Na⁺和K⁺梯度的反转而改变。3. 在无Na⁺培养基中，丙氨酸在粘膜膜上的转运机制与浆膜上一样，具有氨基酸竞争性抑制的相同模式。
