Berton G, Schneider C, Romeo D
Biochim Biophys Acta. 1980;595(1):47-55. doi: 10.1016/0005-2736(80)90246-1.
The effect of some bioflavonoids on the activation of polymorphonuclear leucocyte respiration and exocytosis was examined. At 10-5-10-4 M concentration, quercetin, but not morin and rutin, was found to inhibit the concanavalin A-induced enhancement of oxygen consumption markedly, without impairing leucocyte viability and concanavalin A binding. The inhibition could be reversed by either washing the leucocytes or adding a 10-fold molar excess of 1-anilino-8-naphthalene sulphonate. Concanavalin A-dependent cell secretion of lysozyme was also totally inhibited by 30 muM quercetin. The effect of quercetin on the activation of leucocyte respiration appeared to be stimulus specific. In fact, at a concentration of the flavonoid (75 muM) which provided a 95% inhibition of the concanavalin A-induced stimulation, the respiratory activation produced by phospholipase C was inhibited by about 50% and that caused by myristic acid and by the antibiotic Br-X537A by less than 25%. These data suggest that quercetin exerts its activity at specific sites of the plasma membrane of the leucocytes, and that this compound might be used to identify the membrane domain whereon different stimuli act to originate the initial stimulatory signal.
研究了一些生物类黄酮对多形核白细胞呼吸激活和胞吐作用的影响。在10⁻⁵ - 10⁻⁴ M浓度下，发现槲皮素能显著抑制伴刀豆球蛋白A诱导的耗氧量增加，而桑色素和芦丁则不能，且不影响白细胞活力和伴刀豆球蛋白A结合。通过洗涤白细胞或添加10倍摩尔过量的1-苯胺基-8-萘磺酸盐可逆转这种抑制作用。30 μM槲皮素也完全抑制了伴刀豆球蛋白A依赖性的溶菌酶细胞分泌。槲皮素对白细胞呼吸激活的作用似乎具有刺激特异性。事实上，在该类黄酮浓度（75 μM）下，其对伴刀豆球蛋白A诱导的刺激有95%的抑制作用，而磷脂酶C诱导的呼吸激活被抑制约50%，肉豆蔻酸和抗生素Br-X537A诱导的呼吸激活被抑制不到25%。这些数据表明，槲皮素在白细胞质膜的特定位点发挥作用，并且该化合物可用于鉴定不同刺激作用以产生初始刺激信号的膜结构域。
