Golovenko N Ia, Karaserva T L, Kurushin A A
Vopr Med Khim. 1978 Nov-Dec;24(6):839-45.
Enzymes from mitochondria and soluble fraction of lungs, kidney and liver tissues as well as from blood cells were shown to acetylate 7-aminoderivative of nitrazepame (7-amino-5-phenyl-1-1,2-dihydro-3-N-1,4-benzdiazepine-2-on). The investigation was carried out using thin-layer and column chromatography, UV-spectroscopy and mass spectrometry. Acetylation of the substrate was inhibited in mitochondria but in soluble fraction the reaction was activated by equimolar concentrations of coenzyme A. The acetylating activity towards substrate was 1,000-fold higher in leukocytes than in erythrocytes.
来自线粒体以及肺、肾和肝组织的可溶性部分和血细胞的酶被证明能使硝西泮的7-氨基衍生物（7-氨基-5-苯基-1,2-二氢-3-N-1,4-苯并二氮杂卓-2-酮）乙酰化。使用薄层色谱法、柱色谱法、紫外光谱法和质谱法进行了该研究。在线粒体中底物的乙酰化受到抑制，但在可溶性部分中，等摩尔浓度的辅酶A可激活该反应。白细胞对底物的乙酰化活性比红细胞高1000倍。
