Gleich G J, Adolphson C R, Yunginger J W
J Allergy Clin Immunol. 1980 Jan;65(1):20-8. doi: 10.1016/0091-6749(80)90172-4.
In the performance of the RAST a variety of different solid-phase supports for allergens are utilized. In this study we coupled allergens to microcrystalline cellulose and reduced the quantities of solid-phase allergen tenfold and of anti-IgE fourfold. These reductions actually increased the sensitivity of the RAST for IgE antibodies to several allergens, including those derived from Alternaria tenuis and rye grass pollen. We have termed this procedure the mini-RAST to distinguish it from the procedure employing larger quantities of solid-phase allergen and anti-IgE, the maxi-RAST. We then compared three RAST procedures for the detection of IgE antibodies to ragweed allergens: the maxi-RAST, the mini-RAST, and the paper disk RAST, using both commercial paper disks and disks prepared in our laboratory. The maxi-RAST and the mini-RAST yielded similar results and both appeared more sensitive than the paper disk RAST for the detection of IgE antibodies to ragweed. The results indicate that the microcrystalline cellulose RAST can be significantly scaled down without a loss of its ability to detect IgE antibodies and with a considerable saving of valuable solid-phase allergens and anti-IgE.
在进行放射性变应原吸附试验（RAST）时，会使用多种不同的变应原固相支持物。在本研究中，我们将变应原与微晶纤维素偶联，并将固相变应原的量减少了十倍，抗IgE的量减少了四倍。这些减少实际上提高了RAST对几种变应原IgE抗体的敏感性，包括来自链格孢菌和黑麦草花粉的变应原。我们将此方法称为微型RAST，以区别于使用大量固相变应原和抗IgE的方法，即大型RAST。然后，我们比较了三种检测豚草变应原IgE抗体的RAST方法：大型RAST、微型RAST以及纸盘RAST，纸盘RAST使用了市售纸盘和我们实验室制备的纸盘。大型RAST和微型RAST产生了相似的结果，并且在检测豚草IgE抗体方面，两者似乎都比纸盘RAST更敏感。结果表明，微晶纤维素RAST可以显著缩小规模，而不会丧失其检测IgE抗体的能力，同时还能大量节省宝贵的固相变应原和抗IgE。
