Brown A R, DeWitt C L, Bosma M J, Nisonoff A
J Immunol. 1980 Jan;124(1):250-4.
Dominance of an immune response by secondary cells was demonstrated in a cell transfer system utilizing allotype-congenic mice. Spleen cells from BALB/c mice immunized with TNP-KLH were adoptively transferred into sublethally irradiation (200 R) congenic partners of a different allotype (C.B.-17 mice). Subsequent to the cell transfer, the recipients were challenged with TNP-KLH and bled periodically. Anti-TNP antibodies were isolated and analyzed for their content of donor and host IgG allotypes. Through day 58 after the adoptive transfer, 80% or more of the IgG antibody measured was of donor allotype. By day 110, most but not all recipients made IgG antibody principally of host allotype. In contrast, control C.B-17 recipients, which received nonimmune BALB/c spleen cells but otherwise treated as above, produced IgG antibodies almost entirely of host allotype. Total anti-TNP synthesis was reduced markedly in recipients of immune BALB/c cells so that there was a decrease of about 95% in the production of anti-TNP antibodies of host allotype, as compared with controls that received nonimmune cells. The results are discussed in terms of dominance of an immune response by secondary B cells.
在利用同种异型同基因小鼠的细胞转移系统中，证明了次级细胞对免疫反应的主导作用。用TNP-KLH免疫的BALB/c小鼠的脾细胞被过继转移到亚致死剂量照射（200拉德）的不同同种异型的同基因伙伴（C.B.-17小鼠）体内。细胞转移后，给受体注射TNP-KLH并定期采血。分离抗TNP抗体并分析其供体和宿主IgG同种异型的含量。在过继转移后的第58天，所测IgG抗体的80%或更多是供体同种异型的。到第110天，大多数但不是所有受体产生的IgG抗体主要是宿主同种异型的。相比之下，接受非免疫BALB/c脾细胞但其他处理如上的对照C.B-17受体产生的IgG抗体几乎完全是宿主同种异型的。免疫BALB/c细胞受体的总抗TNP合成明显减少，因此与接受非免疫细胞的对照相比，宿主同种异型抗TNP抗体的产生减少了约95%。根据次级B细胞对免疫反应的主导作用对结果进行了讨论。
