Lauterburg W, Ryffel B, Buerki H, Hess M W, Cottier H, Stoner R D
Blood Cells. 1980;6(1):41-54.
In an attempt to study changing patterns of lymphocyte influx from blood into lymph nodes following regional antigenic stimulation, mice were injected with either fluid tetanus toxoid alone or complexed (at equivalence) with isologous antibody. The mean number of lymphocytes inside the basement membrane of cross-sectioned postcapillary venules (PCV) was evaluated as a function of time after antigen injection. 'Lymphocyte load' of PCV (LL/PCV) began to increase within less than a day after antigenic stimulation. The initial increment of LL/PCV values in both primary and secondary responses was greater when complexed rather than fluid antigen was used. Peak values were reached earlier, i.e., at the end of day 2, in presensitized as compared with unprimed animals (day 4). Maximum values of mean LL/PCV did not reflect the magnitude of subsequent antibody production under all experimental conditions. We conclude that changes in the LL/PCV belong to early lymph node reactions following regional antigenic stimulation. The pattern of this particular response may vary according to the type of immunizing agent used and the state of immunity (sensitization) of the animal as well as other factors still to be elucidated.
为了研究局部抗原刺激后淋巴细胞从血液流入淋巴结的变化模式，给小鼠注射单独的液体破伤风类毒素或与同种抗体结合（等当量）的破伤风类毒素。将横切的毛细血管后微静脉（PCV）基底膜内淋巴细胞的平均数作为抗原注射后时间的函数进行评估。PCV的“淋巴细胞负荷”（LL/PCV）在抗原刺激后不到一天内开始增加。在初次和二次反应中，当使用结合抗原而非液体抗原时，LL/PCV值的初始增量更大。与未致敏动物（第4天）相比，预致敏动物在第2天结束时更早达到峰值。在所有实验条件下，平均LL/PCV的最大值并未反映随后抗体产生的幅度。我们得出结论，LL/PCV的变化属于局部抗原刺激后的早期淋巴结反应。这种特定反应的模式可能因所用免疫剂的类型、动物的免疫状态（致敏）以及其他有待阐明的因素而有所不同。
