Fatori D, Hunter W M
Clin Chim Acta. 1980 Jan 15;100(2):81-90. doi: 10.1016/0009-8981(80)90068-6.
Two variants of a radioimmunoassay for the bipyridylium herbicide Paraquat are described. Both employ antiserum raised to Paraquat-BSA which has been covalently linked to particulate solid-phase support media. The rapid assay for clinical use employs a [3H] Paraquat tracer, requires no agitation and yields results in the range 10--2500 ng/ml serum in 20 min from receipt of sample. The more sensitive assay, designed for research purposes, employs a 125iodinated tracer, requires 2 h continuous agitation but can detect Paraquat at 0.1 ng/ml in simple aqueous solution or 0.25 ng/ml serum. Results from rapid clinical assay agree well with the existing colorimetric method.
本文描述了百草枯二吡啶除草剂的两种放射免疫分析变体。两者均采用针对与颗粒固相支持介质共价连接的百草枯 - 牛血清白蛋白产生的抗血清。临床用快速检测法采用[³H]百草枯示踪剂，无需振荡，从收到样品起20分钟内即可得出10 - 2500 ng/ml血清范围内的结果。为研究目的设计的更灵敏检测法采用¹²⁵碘标记示踪剂，需要连续振荡2小时，但在简单水溶液中能检测到0.1 ng/ml的百草枯，在血清中能检测到0.25 ng/ml。快速临床检测结果与现有的比色法非常吻合。
