胎儿血红蛋白（HbF）γ亚基的分离及其在HbF放射免疫测定中的应用。

The isolation of the gamma subunit of fetal hemoglocin (HbF) and its use in a radioimmunoassay for HbF.

作者信息

James R F, Shuster J, Freedman S O, Gold P

出版信息

J Immunol Methods. 1980;32(3):251-60. doi: 10.1016/0022-1759(80)90190-8.

Abstract

A method is described for the purification, from fetal hemoglobin (HbF), of the fetal specific globin chain (gamma chain) in its native state. In the absence of alpha chain (the globin chain common to all adult human hemoglobins) gamma chain, when used as an immunogen, is able to express its unique antigenicity. Here, a specific, high titer antiserum raised against gamma chain has been used to establish a sensitive radioimmunoassay for HbF. This approach may be applicable to the measurement of other normal and abnormal hemoglobins.

摘要

本文描述了一种从胎儿血红蛋白（HbF）中纯化天然状态下胎儿特异性珠蛋白链（γ链）的方法。在不存在α链（所有成人血红蛋白共有的珠蛋白链）的情况下，γ链用作免疫原时能够表达其独特的抗原性。在此，已使用针对γ链产生的特异性、高滴度抗血清建立了一种用于检测HbF的灵敏放射免疫测定法。该方法可能适用于其他正常和异常血红蛋白的检测。